Click on image to view larger version.
Figure 1. Immunoprecipitation with chaperone antibodies. Immunoprecipitation of 80 µg of microsomal proteins from control (Control) and PiZ transgenic (PiZ) mice and PAGE of the samples were performed as described in Materials and Methods using PDI antibody (Ab)-covered (A and B) or Grp94 Ab-covered (C and D) Sepharose beads. "Sup" refers to the supernatant (not bound proteins) of the immunoprecipitates, whereas "Prec" denotes the proteins remained in the pellet of the immunoprecipitates. Western blot membranes were developed with specific antibodies against the proteins indicated on the right side (A, B, and D) or with a specific Ab against the KDEL sequence (C). Representatives of three independent experiments are presented.
