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Figure 2. TAL and AH activities of the TA increase during Mf differentiation. Mf (2.5x106 cells/well) were cultured in complete RPMI 1640 supplemented with 20% FCS up to 10 days. At the end of the differentiation period, the cells were washed twice with HBSS-10 mM HEPES and the AT, TAL, and AH activities were determined on the total cell homogenates as described in Materials and Methods. Results are means ± SE of 5 separate experiments in duplicate. P values were <0.01 for the TAL and AH activities at day 10 vs. control cells.
