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Figure 3. Schematic diagram of various processes for disruption of AApoAII fibrils. AApoAII amyloidosis has exhibited prion-like transmission. The exogenous AApoAII fibrils can be slightly disaggregated by formalin, delipidation, freeze-thaw, and RNase. The slightly disaggregated fibrils could still induce amyloidosis. Autoclaving, 6 M urea, 2 N NaOH, antibiotics, and NDGA can incompletely disaggregate AApoAII fibrils after which the fibrils induced mild amyloidosis. Autoclaving in 1 N NaOH, formic acid, and guanidine hydrochloride can completely disrupt AApoAII fibrils after which the fibrils did not induce amyloidosis. Accordingly the fibril conformation plays an important role in the transmission of amyloidosis.
