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Figure 1. Phosphorylation of MAPK and Akt by Notch1 activation. A) Endothelial cells transfected with GFP/lenti or NIC-GFP/lenti were restimulated with 50 ng/ml of h-VEGF after starvation for 16 h. Cells were harvested at different time points (as indicated) and lysed for Western blot analyses. Membranes were blotted with antiphospho-Erk1/2 and -Akt Abs and reblotted with antitotal Erk1/2 and Akt Abs. X-ray films were scanned with a densitometer, and specific bands were quantitatively plotted. A representative result is shown. B) Starved HMVECs were treated with sJag1 or sPTPBR7 and restimulated with h-VEGF. Cells were harvested and lysed for Western blot analyses.
