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Figure 1. FAK –/– MEFs were predominately myofibroblasts even after treatment with FGF-2/heparin. FAK +/+, FAK –/–, and DA2 MEFs were treated with 1–20 ng/ml FGF-2 and 5 µg/ml heparin in SSFM for at least 3 days. FAK, (arrows in A, E), was immunodetected in fixed cells with monoclonal anti-FAK followed by secondary goat anti-mouse conjugated to Alexa 488, and SM
A, (arrow heads in D), with mouse anti SMA conjugated to cy3. FAK was immunodetected in FAK +/+ (A) and DA2 MEFs (E), but not in FAK –/– MEFs (C). In contrast, SMA stress fibers were not detected in FAK +/+ MEFs (B) nor DA2 MEFs (F), which express FAK, but were detected in FAK –/– MEFs (D). Scale bars = 10 µm. Percent myofibroblasts: FAK +/+ MEFs: 2% ± 3%; FAK –/– MEFs: 95% ± 7%; DA2: 3% ± 5%. Values are means from 4 independent experiments ± SD P < 0.05 FAK –/– compared with FAK +/+, and P < 0.05 FAK –/– compared with DA2.
