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Figure 1. Peroxidase activity of human recombinant Grx2. A) SDS-PAGE analysis of purified recombinant human and mouse Grx2. Lane 1, MW marker; lane 2, human Grx2; lane 3, mouse Grx2. B) GSH-dependent peroxidase activity of Grx2. C) Inhibition of GSH-dependent peroxidase activity by anti-Grx2 antibody (Ab). D) TR-dependent peroxidase activity of Grx2. E) Inhibition of TR-dependent peroxidase activity by anti-Grx2 Ab. Error bars indicate SD, n = 5. Asterisk indicates significant (P<0.01) difference from the control group with nonspecific IgG, determined by Dunnett’s test after significant ANOVA.
