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Figure 2. Akt is involved in cannabinoid-induced inhibition of melanoma cell proliferation. A) B16 cells were cultured for the times indicated with vehicle, 100 nM WIN-55,212–2, 1 µM THC, or 100 nM JWH-133; extracts were obtained, and Western blot analysis was performed. One representative experiment is shown. OD values (in percentage of the respective vehicle incubations; n=4) are given for phospho-Akt relative to total Akt in short-term incubations. *Significantly different (P<0.01) from vehicle incubations. B, C) B16 cells were infected with HA-Akt or empty vector and further cultured for 48 h with vehicle or 100 nM WIN-55,212–2. The number of viable cells (B) and pRb phosphorylation status (C) were determined. Western blot controls of Akt expression in the different infection conditions and OD values for pRb are shown. Results correspond to four different experiments. *Significantly different (P<0.01) from vehicle incubations.
