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Figure 1. Cannabinoid administration inhibits melanoma progression and metastatic spreading in mice. A) Tumors were generated by s.c. injection of B16 cells in C57BL/6 mice, and animals were treated with either vehicle or cannabinoid (either WIN-55,212–2 or JWH-133 at 50 µg/day, daily) for up to 8 days (n=8 for each experimental group). Tumor size was monitored during the treatment. Examples of tumors in the flank of mice and after dissection are shown. *Significantly different (P<0.01) from vehicle administration. B) Tumors were generated by s.c. injection of B16 cells in nude mice, and animals were treated with either vehicle or WIN-55,212–2 (50 µg/day, daily) for 8 days (n=6 for each experimental group). *Significantly different (P<0.01) from vehicle administration. C) B16 cells were injected intraplantarly in C57BL/6 and nude mice, and animals were treated with either vehicle or WIN-55,212–2 (50 µg/day, every 3 days) for 21 days (C57BL/6 mice, n=6) or 12 days (nude mice, n=5). The number of metastatic nodules in the lungs and the liver were subsequently counted. Significantly different (#P<0.05, *P<0.01) from vehicle administration.
