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Figure 2. Comparison of uhCG-induced angiogenic effect between WT and LuRKO (KO) mice in the aortic ring assay. LuRKO mice aortic rings were cultured in a collagen gel during 9 d in presence of autologous sera (2%) and/or urinary hCG (100 IU/ml).
A) Photomicrographs (original magnification 25x) of aortic rings from WT mice and LuRKO mice after incubation with autologous sera and supplementation with uhCG (100 IU/ml). B) Quantification of microvessel outgrowth (Nv) by computerized image analysis of aortic ring assay after 9 d of incubation with autologous sera supplemented or not with uhCG (100 IU/ml) in WT or KO mice aortics. sWT = WT mice serum. sKO = KO mice serum. Data are expressed as mean ± SD, n = 5, *P < 0.05. Experiments were repeated at least three times. Differences between the experimental conditions were evaluated using the ANOVA analysis (P values <0.05 were considered significant).
