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Figure 2. Eap interferes with ERK1/2 activation. Serum-deprived HUVEC were left untreated or incubated with 100 µg/ml Eap for 1 h before stimulation with 100 ng/ml of either VEGF (A) for the times indicated. Each lane contains 50 µg of total cell protein; blots were tested with antibodies specific for the phosphorylated forms of either ERK1/2, p38, or Akt. HUVEC or BREC (B) were incubated with increasing concentrations of Eap, as indicated.
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