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Figure 2. GPCR agonists induce the translocation of PP2A to the membrane fraction and its coimmunoprecipitation with the Na,K-ATPase 
1-subunit. A) ATII cells were incubated with 10 µM dopamine for the indicated times, 1% Nonidet P-40 soluble fraction was isolated and a Western blot using an Ab against the catalytic subunit of PP2A was performed. Upper panel) Shows a composite graph of four different experiments. Lower panel) Depicts a representative Western blot. B) GFP1-A549 and GFP-S18A1-A549 cells were exposed for 0 and 15 s to 10 µM dopamine. An immunoprecipiation with and anti-GFP Ab and Western blot against PP2Ac were performed (upper panel). Equal loading was confirmed stripping the membrane and performing a Western blot against GFP (lower panel). DA: dopamine; i.b.: immunoblot. *P < 0.05.
