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Figure 1. GC-resistance is associated with reduced induction of GR and its downstream target GILZ. A) GC-sensitive (open circles) and GC-resistant (closed triangles) CEM-C7H2 subclones (top panels) and the GC-sensitive parental PreB697 (open circle) and its GC-resistant subclones (closed triangles, bottom panels) were left untreated (–) or treated (+) with 10–7M dexamethasone for 6 or 24 h as indicated before determining GR and GILZ expression by quantitative real-time reverse-transcriptase polymerase chain reaction (RT-PCR). Expression levels of GR and GILZ normalized to TBP are shown in arbitrary units. B) The indicated cell lines were treated with 10–7M dexamethasone or 0.1% ethanol (as carrier control) and subjected to Western blotting using antibodies against GR or GAPDH as a loading control.
