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Figure 2. A) Amino acid sequence identity within the EGF domains of CD97 and EMR2 between species from different mammalian orders. Numbers are mean values. CD97 sequences were compared between primates (human), rodents (mouse, rat), hoofed animals (cow, pig), and carnivores (dog). Conservation of EGF domain 4 (in boldface) is significantly higher (P<0.05) than in any other EGF domain. EMR2 sequences were compared among primates (human, rhesus macaque) and carnivores (dog). B) Transcription of CD97 and EMR2 isoforms in peripheral blood leukocytes of the dog. RT-PCR was performed with primers that amplify the EGF domain region-encoding sequences of CD97 and EMR2. C) Binding of canine EMR2 to CS. Shown is a representative experiment that measures binding of fluorescent probes, loaded with the extracellular region of the respective EMR2 isoforms, to CHO cell mutants that either fail to express glycosaminoglycans (PgsB-618, left panel) or express exclusively CS (PgsD-677, right panel). The nonbinding human EMR2–2EGF and human EMR2–5EGF, known to bind CS, have been included as negative and positive control, respectively. c, dog; h, human.
