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Figure 2. Effects of acute immunization with anti-Aß Ab on Aß levels in the brains of Tg2576 mice; 3 month old Tg2576 mice were dosed with 500 µg biotinylated mAb9. 6 h–14 days later mice were perfused with PBS and Aß levels in GuHCl (A), TBS (B), and RIPA (C and D) brain extracts were detected by ELISA using end-specific anti-Aß40 mAb40.1 or anti-Aß42 mAb42.2 as capture and 4G8-HRP as detection; n = 4 per group. E) 3 month old Tg2576 mice were dosed with 500 µg biotinylated mAb9 every week for 4 wk. Mice were killed 24 h after the final mAb admisntration. Aß levels in RIPA brain extracts were detected by ELISA; n = 4 per group.
