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Figure 1. Aß and mAb levels in plasma of Tg2576 mice after acute passive immunization. A) 3 month old Tg2576 mice were dosed ip with 500 µg (1600 pmol) biotinylated mAb9. Aß levels (A) or levels of Aß bound by biotinylated mAb9 (B) were measured at different time points by ELISA. C) Plasma from Tg2576 mice dosed with biotinylated mAb9 or biotinylated mouse IgG was fractionated by size-exclusion chromatography. Levels of Aß in each fraction were measured by ELISA. D) Aß:biotinylated mAb complex in the plasma of treated Tg2576 mice was immunoprecipitated with streptavidin beads and Aß was detected by mAb9. E and F) 3 month old nontransgenic mice were dosed ip with 500 µg (1600 pmol) biotinylated Ab9 (E) or with complex of 
1600 pmol biotinylated mAb9 and 3200 pmol Aß40 (F). Biotinylated mAb9 levels (E) or levels of Aß bound by biotinylated mAb (F) in plasma were measured by direct ELISA; n = 4 per group, *P < 0.001, **P < 0.01 vs. control.
