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Figure 2. PCCl3 cell growth is regulated by agonist stimulation of PLA2IV
and PLA2IV-derived products. A) [3H]-Thymidine incorporation in cells stimulated for 48 h with ATP (100 µM) and NE (20 µM) without and with pyrrophenone (0.5 µM), GroPIns (100 µM, 400 µM), LysoPdtIns (10 µM), arachidonic acid (AA, 1 µM), and TSH (10–10 M) (means±SE; 5 independent experiments). **Significantly different from respective controls (P
0.01). B) Representative growth curves of stable Vclone (empty vector), WTclone (PLA2IVwt), and DNclone (PLA2IV1–552) PCCl3 transfectants (means±SE; single experiment in triplicate of 3 independent experiments). C) Cell growth of WTclone in the absence (–) and presence (+) of pyrrophenone (0.1 µM) (means±SE; 3 independent experiments).
