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Figure 2. In response to high Cao2+, NF-
B moves from the cytoplasm (A) to the nucleus (B) in mature rabbit osteoclasts. Maximum translocation is observed at 1 h, when 61 ± 7% of the osteoclasts exhibit nuclear localization of NF-B. Nuclear localization of NF-B then slowly decreases to reach a plateau at 6 h (
25%), when NF-B activation remains significantly different from that observed in osteoclasts cultured for 1 h in the presence of 1.8 mM Cao2+ (P<0.01). When DN-CaR-transfected rabbit osteoclasts were treated with 20 mM Cao2+ for up to 12 h, Cao2+-induced activation of NF-B was dramatically reduced compared with ß-Gal-transfected osteoclasts (C).
