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Figure 2. In vivo survival, differentiation and function of Nurr1-induced DA cells. A–F) Representative confocal images stacks (35 µm in z) of the TH+ striatal grafts at 4 wk after transplantation derived from precursors transduced with N (A, D), NHB (B, E) and NM (C, F). The boxed areas of (A–C) are magnified in (D–F). Scale bar, 20 µm. G) Quantification of TH+ cells in grafts at 4 and 8 wk after transplantation. *Significantly different from N-grafted at P < 0.05, n = 5 for each value. H) DA levels in grafts 8 wk after transplantation. *P < 0.05, n = 5 for each value. Behavioral recovery was assessed by amphetamine-induced rotation (I) and step adjustment tests (J) in shame-operated (PBS-injection, n=8), or animals grafted with precursors transduced with N (n=10), NHB (n=25), and NM (n=15). *Significantly different from shame-operated at P < 0.05.
