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Figure 1. Expression of the mature neuron marker MAP2 in Nurr1-induced TH cells. Immunocytochemistry was carried out at day 10 of in vitro differentiation in the cultures transduced with Nurr1 alone (N), Nurr1+SHH+Bcl-XL (NHB), and Nurr1+Mash1 (NM). Note that TH+ cells in NHB and NM-cultures exhibit mature neuronal morphologies with multiple processes. Graph D represents the percentages of TH+ cells coexpressing MAP2 out of total TH+ cells *Significantly different from N control with the P value of <0.001. Scale bar, 10 µm.
