Click on image to view larger version.
Figure 1. Cellular localization of hGX sPLA2 in human carotid arteries containing atherosclerotic plaques. Sections of arteries were stained with anti-CD68 (green, A and D), anti-hGX sPLA2 (red, A–D) and anti 
-actin (green, B and C). Double immunofluorescence confocal analysis was performed and colocalized regions are shown in white. In intima (A and B), hGX sPLA2 expression is colocalized with foam cells (A) and smooth muscle cells resembling myofibroblasts (B). Inserts represent colocalization plots between hGX sPLA2 (red) and specific cell markers cluster of differentiation (CD) 68, or -actin, (green). Blue dots represent colocalization points. In media (C), hGX sPLA2 expression is localized to ECM. No colocalization with smooth muscle cells (stained with -actin, green) of media was observed. In intima (D), incubation of sections with 1 µM recombinant hGX sPLA2 (rec hGX) abolished the hGX sPLA2 labeling.
