Click on image to view larger version.
Figure 2. Effect of 25-(OH)D3 incubation on human osteoblast gene expression and osteoblast function. Human pre-osteoblasts were induced to differentiate and at various time points during differentiation treated for 48 h with 1000 nM 25-(OH)D3 (
), 10 nM 1
,25-(OH)2D3 (), or vehicle (
), then analyzed for effects on A) OC mRNA expression by real-time PCR and B) ALP activity. C) OC mRNA expression was measured after incubation with various concentrations of 25-(OH)D3 and 100 µM of ketoconazole for 24 h on day 14 of SV-HFO culture. Data are shown as means ± SD. *P < 0.05 and **P < 0.01 vs. control on the same day.
