FASEB J.
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Figure 1


Figure 1. Activity of 1{alpha}-hydroxylase in human osteoblasts.

A) The human osteoblast cell lines SV-HFO and MG-63 were incubated for 48 h with 1000 nM 25-(OH)D3 and next production of 1{alpha},25-(OH)2D3 was measured. B) SV-HFO cells were incubated with various concentrations of 25-(OH)D3 for 48 h in the absence (•) or presence ({square}) of 100 µM ketoconazole after which 1{alpha},25-(OH)2D3 production was measured. Data shown are means ± SD.





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