FASEB J.
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Table 2. Corroboration of mRNA gene transcripts at the level of protein expression

Molecule RNA hybridization signals (Fold increase over controls) Protein assays confirming RNA-based assessments (fold increase over controls)
2 h 24 h Assay 6 h 24 h

IL-6 57,600* (12x) 40,800 (8.1x) ELISA (12.5x)
TNF{alpha} 65,800 (48x) 3048 ELISA (62.5x)
(2.2x)
ICAM1 25,800 6700 FACS{dagger} (100x)
(7.8x) (2.01x)
SLAM 1,600 2500 FACS (58x)
(24x) (34x)
LY9 3500 7000 FACS (11x)
(2.4x) (4.8x)
41BBL 11,000 1900 FACS (7x)
(27x) (4.2x)
FLIP 7800 3500 Western blot Increased
(5.1x) (2.3x) (nd{ddagger})
CCR7 20,000 31,000 Western Increased
(1.7x) (2.7x) Blot (nd)

* Average hybridization signal of three independent samples of DC treated with B7-DC XAb.

{dagger} Mean fluorescent intensities of antibody stained DC estimated by flow cytometry.

{ddagger} Fold-change not determined.





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