| Molecule |
RNA hybridization signals (Fold increase over controls) |
|
Protein assays confirming RNA-based assessments (fold increase over controls) |
|
|
|
2 h |
24 h |
Assay |
6 h |
24 h |
|
| IL-6 |
57,600* (12x) |
40,800 (8.1x) |
ELISA |
|
(12.5x) |
TNF |
65,800 (48x) |
3048 |
ELISA |
|
(62.5x) |
|
|
(2.2x) |
|
|
|
| ICAM1 |
25,800 |
6700 |
FACS |
(100x) |
|
|
(7.8x) |
(2.01x) |
|
|
|
| SLAM |
1,600 |
2500 |
FACS |
(58x) |
|
|
(24x) |
(34x) |
|
|
|
| LY9 |
3500 |
7000 |
FACS |
(11x) |
|
|
(2.4x) |
(4.8x) |
|
|
|
| 41BBL |
11,000 |
1900 |
FACS |
(7x) |
|
|
(27x) |
(4.2x) |
|
|
|
| FLIP |
7800 |
3500 |
Western blot |
|
Increased |
|
(5.1x) |
(2.3x) |
|
|
(nd ) |
| CCR7 |
20,000 |
31,000 |
Western |
|
Increased |
|
(1.7x) |
(2.7x) |
Blot |
|
(nd) |
|