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Figure 1. NanoTiO2 nanoparticle-induced pulmonary morphological and histological changes. A) Morphological (a, b) and histological (c, d) changes (H/E, hematoxylin and eosin staining) in the mouse lung 1 wk after intratracheal instillation with NS (normal saline, a, c) or 0.1 mg/mouse nanoTiO2 (b, d). Arrowheads indicate the nodule-like lesions caused by chronic inflammation. Original magnification x100, bar = 100 µm for H/E histological image (c, d). B) Histological changes in the mouse lung after intratracheal instillation with nanoTiO2 for 1 wk. Lung tissues were collected from NS-treated control mice (a) and nanoTiO2 (0.1 mg/mouse) -treated mice (b, c). Original magnification, x400, bar = 50 µm. Similar results were obtained in 6 independent experiments. C) Morphometric measurements from lungs at 3 days, 1 wk, and 2 wk after installation with NS or nanoTiO2 (0.1 or 0.5 mg/mouse). The mean linear intercept (MLI), mean air space area, and septal chord length were measured (n=6) as described in Materials and Methods. The data are the means ±SD. *P < 0.05 in Student’s t test compared with the control (NS) group.
