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Figure 1. Expression of hClC-4-GFP transfected in vivo into murine skeletal muscles. Anterior tibial muscles of C57BL/10 mice were dissected 2–5 days after intramuscular electrotransfer of the hClC-4-GFP plasmid and cryosections imaged by confocal microscopy. A) ClC-4-GFP signal (green) shows a reticular pattern throughout the muscle fiber, with a higher concentration near the transfected myonuclei; dimension bar = 50 µm. B) Left top: ClC-4-GFP signal (green) in a section of muscle fiber. Left bottom: the same section stained for actin-myosin skeletal muscle fibers with phalloidin (red). Right: overlay of the GFP and phalloidin signals (yellow). Dimensional bars = 25 µm. C) Left top: ClC-4-GFP signal (green) in a section of muscle fiber. Left bottom: same section stained for SERCA (red). Right: overlay of the GFP and SERCA signals (yellow). Dimension bars = 25 µm. D) Left top: ClC-4-GFP signal (green) in a section of muscle fiber. Left bottom: the same section stained for ryanodine receptor (red). Right: overlay of the GFP and ryanodine receptor signals (yellow). Dimension bars = 25 µm.
