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Figure 1. Effect of ECD
2 on IL-13- or IL-4-induced STAT6 phosphorylation in THP-1 and COS-7 cell lines. Cells were incubated with 50 ng/ml IL-13 or IL-4 in the presence of 0, 500, or 2000 ng/ml of ECD2-EC (A) or 0, 25, 50, or 100 ng/ml ECD2-His6 (B) for 15 min. C) Cells were incubated with various concentrations (0–100 ng/ml) of IL-13 or IL-4 with 12.5 or 100 ng/ml ECD2-His6, respectively. D) Band intensity was measured using National Institutes of Health-Image 1.67 to determine the inhibitory effect of ECD2-His6 on IL-13-induced STAT6 phosphorylation. E) Cells were incubated with 50 ng/ml IL-13 in the presence of 0, 50, or 500 ng/ml ECD2-His6 that was or was not deglycosylated by PNGase F. Cells were lysed, and Western blotting analysis was performed.
