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Figure 1. Mycetomes and Malphigian tubule of L. bostrychophila and transovarial transmission of Rickettsia. A–C) Mycetomes of adults. Visualization of different mycetocytes that form the mycetome. Free rickettsial cells surround mycetome. C) 3D projection of 20 µm scanning, sagittal view of panel A showing thickness of the mycetome. D) Heavy infection of Malpighian tubule and surroundings in adult. E) Duplication of the mycetome in teneral. F) Horizontal section, ventral view of teneral of picture A showing the position of mycetomes at both sides of the body (arrows), the duplicated mycetome on the left side. G) Horizontal section. Transmission of Rickettsia into growing oocyte. Visualization of infection in the posterior base (arrow) of the nurse cells where transmission of Rickettsia into the growing oocyte occurs (circle). The Rickettsia clusters commence surrounding the oocyte nucleus. Follicular cells are free of infection. Fat body contains localized infected cells and the midgut epithelium shows a uniform distribution. Gut lumen is free of bacteria. NC, nurse cells; NU, oocyte’s nucleus; FC, follicular cells; MGE, midgut epithelium; GL, gut lumen; OO, oocyte. Bars 20 µm.
