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Figure 1. Oncostatin M inhibits NMDA-mediated excitotoxic injury in vitro. A) Cortical neurons were incubated in the absence or presence of 50 µM NMDA and/or 50 ng/ml oncostatin M (OsM) and the degree of cell death determined 24 h later using the MTS cell viability assay. Percent viability was determined relative to untreated (control) cells. *Significant difference (P<0.01; n=3) compared to cells treated with NMDA alone. B) Cortical neurons were left untreated or pretreated with either 50 or 10 ng/ml OsM for 24 h. Culture media were then replaced with either fresh media or OsM (50 or 10 ng/ml) together with NMDA (50 µM) as indicated, and the degree of cell death was determined 24 h later using the MTS cell viability assay. Percent viability was determined relative to untreated (control) cells and results presented are mean values ±SD of quadruplicate determinations from 3 independent experiments. *P < 0.001 and **P < 0.01, significant difference compared to cells treated with NMDA alone.
