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Figure 2. Differential effect of 1,25(OH)2D3 and ZK191784 on 45Ca2+ uptake in the intestinal Caco-2 cell line and on transepithelial Ca2+ transport in rabbit kidney CNT/CCD primary cell cultures. 45Ca2+ uptake was determined in Caco-2 cells incubated for 48 h in normal culture medium (Control) or culture medium supplemented with 1 · 10–7 M 1,25(OH)2D3, 1 · 10–7 M ZK191784 or 1 · 10–7 M 1,25(OH)2D3 together with 1 · 10–7 M ZK191784, respectively (A). Data are depicted as ruthenium red (RR)-sensitive uptake. Transcellular Ca2+ transport was determined in immunodissected rabbit CNT/CCD cultures incubated for 48 h in normal culture medium (Control), culture medium supplemented with 1 · 10–7 M 1,25(OH)2D3, 1 · 10–7 M ZK191784, or 1 · 10–7 M 1,25(OH)2D3 together with 1 · 10–7 M ZK191784 (B). Data are mean ± SE. *P < 0.05 vs. untreated cells (Control); #P < 0.05 vs. 1,25(OH)2D3-treated cells.
