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Figure 1. Renal Ca2+ excretion and intestinal Ca2+ absorption during treatment with ZK191784 in TRPV5+/+ and TRPV5–/– mice. Effect of ZK191784 on renal Ca2+ excretion was determined in metabolic cages (A). Intestinal Ca2+ absorption was determined in an in vivo 45Ca2+ absorption assay, measuring serum 45Ca2+ at early time points after oral gavage (B). Controls, mice treated with vehicle only; ZK191784, mice treated for 28 days with the 1,25(OH)2D3 analog ZK191784 (50 µg/kg/day). 
= TRPV5+/+ controls, treated with vehicle only; 
= ZK191784-treated TRPV5+/+; 
= TRPV5–/– controls, treated with vehicle only; 
= ZK191784-treated TRPV5–/–. Data are mean ± SE. *P < 0.05 vs. TRPV5+/+ controls; #P < 0.05 vs. TRPV5–/– controls; n = 9 animals per group.
