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Figure 1. qFRET measurements of the interactions of K141EHSP22 and K141NHSP22 with wtHSP22 and with themselves in doubly transfected COS-7 cells 24 h after transfection. The AAFE was determined in cells expressing the various HSP22 species as indicated. The HSP22 species were used as CIT (top row of the abscissa legend) and CFP fusion proteins (bottom row of the abscissa legend). All sample values were significantly different from the negative control (expression of CIT and CFP). Significant differences from the wtHSP22/wtHSP22 (positive control) interaction (*), from the wtHSP22/K141EHSP22 interaction (+), and from the K141EHSP22/K141EHSP22 interaction (x) are indicated where appropriate. Abbreviations: C, control; wt, wtHSP22; E, K141EHSP22; N, K141NHSP22.
