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Figure 2. Clinical and physiological changes in transgenic mice. A, B) Histograms of isometric limb strength demonstrating weakness in forelimb and hind limb of transgenic mice, respectively (n=13 Tg and 10 non-Tg). C) Waveforms indicating typical changes in MUAP in skeletal muscle of transgenic mice (middle and lower traces) compared with nontransgenic littermates (top trace). D, E) Frequency histogram of MUAP amplitude and area, respectively. Inserts show mean MUAP amplitude (D) and area (E) (n=3 Tg and 3 non-Tg). F) Changes in membrane potential and resting [Ca2+]i (black bar, Tg; open bar, non-Tg). G) Plot of individual fiber Vm. Using a non-Tg upper cutoff of –80 mV (Vm), two subpopulations of Tg (1 and 2) according to Vm are created. H) Graph showing changes in resting [Ca2+]i and the corresponding distribution of transgene-bearing fibers into two populations according to the cutoff established in panel G (n=3 Tg and 3 non-Tg animals, all Tg are from the same line). *Significantly different from control (P<0.05), independent t test, mean ± SE. See full text for quantification of muscle injury markers.
