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Figure 1. Histological changes in skeletal muscle of MLC-ßAPP mice. A) Serial sections of tibialis anterior showing immunoreactive ßAPP deposits (arrows). B) Inclusion-like profiles under phase contrast imaging. C) Aß immunoreactivity and D) Gomori trichrome staining showing corresponding inclusion and/or tubular aggregate formation. E) Serial sections of triceps showing ßAPP immunoreactivity in fast twitch fibers are identified in panel F by negative or light ATPase (pH 4.6) staining. G) Gomori trichrome histochemistry identifies clefted inclusions (arrow) and a relatively light cytosolic reaction in an abnormal myofiber adjacent to a neurovascular bundle. H) Serial sections of hamstring showing intramyofiber Aß immunoreactivity. I) Corresponding fast twitch fibers identified by negative ATPase (pH 4.6) stain (arrow) and by J) positive immunoreactivity for fast myosin heavy chain (arrow). K–N) Serial sections of hamstring demonstrating immunoreactive Aß1–42 deposits using specific antibodies, Chemicon (K) and 21F12 (M). The corresponding thioflavin S reactions are given in panels L and N, respectively. O, P) Histological development with hematoxylin and eosin reveal centralized nuclei in transgenic mice compared with nontransgenic littermate, respectively. See full text for ultrastructural studies.
