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Figure 2. ApoCI associates with increased inflammatory response and survival of mice with experimental sepsis. A–D) Apoc1–/– (n=13), WT (n=15), and APOC1 mice (n=11) were inoculated intratracheally with 500–750 colony forming units of Klebsiella pneumoniae. A–C) After 24 h, plasma levels of TNF-
(pooled plasma, immunoblot), MIF (individual mice, immunoblot), and E-selectin (individual mice, ELISA) were determined. *P < 0.05, **P < 0.01. Insets show immunoblots of pooled plasma (D). After 48 h, plasma levels of Klebsiella pneumoniae were determined. **P < 0.01. E) Apoc1–/– (•, n=25), WT (
, n=29), and APOC1 (
, n=19) mice (i.e., including mice described above and a second set of mice) were inoculated intratracheally with same dose of Klebsiella pneumoniae or saline (
, WT mice, n=6) and their 2-wk survival was assessed. *P < 0.05, **P < 0.01 (log-rank test, Graph Pad Software Inc.).
