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Figure 1. ApoCI enhances LPS-triggered TNF-
induction. A) LPS (25 µg/kg) was injected into apoc1–/–, WT, or APOC1 mice (n=6), and TNF- was determined in plasma after 60 min. B-D) RAW 264.7 cells were incubated (4 h at 37°C) with ReLPS (1 ng/ml; B), wtLPS (1 ng/ml; C), or zymosan (ZS; 100 ng/ml; D) in DMEM supplemented with 0.01% human SA, without or with preincubation (30 min at 37°C) with apoCI, apoCImut, or apoCIII (molar ratio apo:ReLPS=10:1; apo:wtLPS=100:1; apo:zymosan=3450:1), and TNF- was determined in medium (n=4). *P < 0.05, **P < 0.01; nd, not detected.
