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Figure 1. Cooperative production of cytokines in cocultures. Airway smooth muscle cells (ASMCs) or BEAS-2B epithelial cells were grown to confluence in 24-well plates and stimulated with media, poly(I:C) (25 µg/ml), LPS (10 ng/ml), or poly(I:C) + LPS in the presence or absence of PBMCs (10,000 cells per well). After 24 h, levels of CXCL8 and CXCL10 in the media were determined by ELISA. Poly(I:C) caused production of CXCL8 and CXL10 from monocultures of ASMCs, whereas LPS activated ASMCs or BEAS-2B cells effectively only in the presence of PBMC. Stimulation with both agonists enhanced the production of cytokines from PBMC/tissue cell cocultures. A, B) Effects of the indicated agonists and culture conditions on CXCL8 and CXCL10 release from ASMCs; C) the effects of these stimuli on CXCL8 generation from BEAS-2B cells. In each graph, open bars show cytokine generation from monolayers of the relevant tissue cell (ASMCs or BEAS-2B cells), shaded bars show cytokine generation from wells containing 10,000 PBMCs only, and filled bars show cytokine generation from cocultures of PBMCs and the indicated tissue cell. Data shown are from 4 passages of BEAS-2B cells and from 5 passages of one ASMC donor. Selected relevant comparisons were performed using Student’s t test and the results indicated.
