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Figure 2. Sema 3C induces MGEC directional migration, capillary-like network formation, and VEGF-A secretion. A) Sema 3C (500 ng/ml) induced a 44 ± 11% increase in MGEC migration at 3 h. Data are mean ± SE cell counts from 3 independent experiments performed in duplicate. *P < 0.05 sema 3C vs. control; B) MEGC plated on collagen I gels in serum free medium for 48 h showing minimal network formation; C) MEGC plated on collagen I gels and exposed to sema 3C (360 ng/ml) for 48 h developed extensive capillary-like network. Images are representative of 3 independent experiments performed in triplicate. D) Western blot showing VEGF164 expression in control and sema 3C treated MGEC cell lysates and VEGF120 secretion to the supernatant, demonstrating that sema 3C induces significant VEGF120 secretion. E) Time course of sema 3C-induced VEGF120 secretion. F) Western blot showing that VEGF120 secretion is not mediated by 
5 ß1 or V ß3 integrins. G) Western blot showing that VEGF120 secretion is mediated by NP and NP2 signaling. All Western blots are representative of 
3 separate experiments.
