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Figure 2. Decreased CAK phosphorylation of PML/RAR
occurs only when MAT1 levels are reduced. Autoradiography showed that, in the presence of constant levels of GST-PML/RAR, CAK activity in phosphorylation of GST-PML/RAR was inhibited when MAT1 levels were reduced after 48 h of ATRA treatment. The phosphorylation of CAK-bound PML/RAR in the precipitates was also similarly diminished on ATRA-induced MAT1 reduction. The autoradiography blot was probed with different antibodies as indicated. GST-PML/RAR (lane 9) was used as a positive control in Western blot analysis. P-K7, hyperphosphorylated form of CDK7; K7, hypophosphorylated form of CDK7.
