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Figure 1. CAK-PML/RAR
dissociation precedes MAT1 degradation and reduction of CAK abundance in ATRA-induced P/D transition. A and B) PML/RAR was precipitated by anti-CDK7 Ab from NB4 cells exposed to ATRA for up to 12 h (A) or for up to 120 h (B). The precipitates were then probed with anti-RAR and anti-MAT1 antibodies. C) Either 1 or 5 µM of ATRA showed similar inhibitory effect on NB4 cell proliferation. The growth curves represent the means ± SD from the cells of triplicate wells. D) pRb expression and phosphorylation status were depicted by Western blot analysis in ATRA-induced P/D transition. P-pRb, hyperphosphorylated form of pRb; pRb, hypophosphorylated form of pRb. E) nuclear segmentation of NB4 cells treated with ATRA. F) CD11b expression following ATRA stimuli.
