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Figure 1. ASPA in cultured oligodendrocytes. A) ASPA staining in a representative mature cultured rat oligodendrocyte using an Ab against recombinant murine ASPA (
ASPA) (red). Cell processes are highlighted using an Ab against myelin oligodendrocyte protein (MOG) (green). Nuclei are stained with DAPI (blue). Merged field shows that ASPA colocalizes with DAPI. Scale bar = 20 µm. B) ASPA staining in cultured murine oligodendrocytes using serum against a conserved ASPA peptide (pepASPA) (green). Nuclei are stained with DAPI (blue). Cytoplasm is stained with CC1 oligodendrocyte marker protein (red). Top panel) White arrows point to examples of cells where CC1 and ASPA staining correlate very weakly. Bottom panel) Perinuclear staining examples in mostly differentiated oligodendrocytes are depicted with green arrows. An example of predominantly nuclear ASPA staining is marked by a white arrow. Merged field shows ASPA colocalizing with DAPI. Top panel: scale bar = 100 µm. Bottom panel) Scale bar = 20 µm.
