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Figure 2. Genistein activates extracellular-signal regulated kinase pathway. A representative Western blot is shown of phospho-extracellular-signal regulated kinase (ERK) 1/2 in MCF-7 cells after 3 min incubation with genistein (0.5 µM) alone or following coincubation with 1 µM U0126. Histograms represent densitometric measurement of specific bands of phospho-ERK 1/2 content using total ERK levels as housekeeping controls. Data are expressed as means ± SD for 5 independent experiments, *P < 0.05; **P < 0.01 vs. control.