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Figure 2. Effects of modifying eIF4F activity on the flux of c-jun mRNA. Cardiocytes were infected for 4 h with the indicated adenoviruses at a moi of 4. After a 48 h incubation period, cardiocytes were challenged with TPA for 1 h. Relative levels of c-jun mRNA and 18S rRNA were measured by real-time RT-PCR in the total RNA fraction and in the pooled polysome fractions. The c-jun mRNA/18S rRNA ratios are expressed as the fold increase over nontreated controls. *P < 0.03 as determined by Student’s t test. The numbers of experiments for each group are indicated.
