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Figure 1. Functional distribution of c-jun mRNA in adult cardiocytes. Cardiocytes were challenged with 200 nM TPA for either 1 or 4 h prior to fractionation of homogenates on linear sucrose gradients. The levels of c-jun mRNA and GAPDH mRNA were measured in the total RNA fraction and in the polysome bound RNA fractions by real-time polymerase chain reaction. Fold-increases in mRNA levels following TPA challenge were calculated relative to the nontreated controls. Values are the mean ± SE. *P < 0.05 as determined by Student’s t test.
