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Figure 1. Time course of STAT1 and STAT3 phosphorylation after ischemia/reperfusion injury. Protein lysates from heart were separated on SDS-PAGE gels and probed with antibodies for A) total STAT1, phospho STAT1Y701, and phospho STAT1S727 and B) total STAT3, phospho STAT3Y705, and phospho STAT3727. Equal protein loading was confirmed by probing with an antibody (Ab) to GAPDH.
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