Supplemental Data

*NOTE: .mov files must be viewed with QuickTime Player Version 7.1.3*

Files in this Data Supplement:

• Supplemental Figure 1 - (SuppFig1.jpg; 361 KB) Life cycle of the periodical cicada. After 17 years underground, cicadas emerge en masse as nymphs and morph into flying insects. Serenading males attract females to reproduce and, following fertilization, females deposit eggs with a saw-like ovipositor into multiple sites beneath the bark of small twigs and branches. After ~2 months, the eggs hatch into ant-like nymphs, fall to the ground and rapidly burrow into the soil.
• Supplemental Movie 2 - (SuppMovie2.mov; 704 KB) Mating call of the male M. cassini consists of a series of small ticks followed by a single undulated buzz. The buzz is only shown in this movie clip.
• Supplemental Movie 3 - (SuppMovie3.mov; 1.64 MB) Mating call of the male M. septendecula. Males produce short chirps with each string of chirps lasting 10–30 sec.
• Supplemental Movie 4 - (SuppMovie4.mov; 7.0 MB) Male and female cicadas mate over a period of ~1 hr. Following fertilization, females deposit their eggs under the bark of twigs or small branches.
• Supplemental Movie 5 - (SuppMovie5.mpg; 3.57 MB) Animated three-dimensional microCT view of the periodical cicada showing the tymbal muscle highlighted in yellow (rotated around the dorsal-ventral axis).
• Supplemental Figure 6 - (SuppFig6.gif; 29 KB) Tymbal muscles contract alternately and pull via an apodeme tendon on the tymbal, deflecting the tymbal inward and producing a resonance within the hollow abdomen. Upon muscle relaxation, the tymbal 'pops' outward.