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Figure 1. TACE-induced RIP of p75NTR and suppression of Rho and EGFR activation in the presence/absence of CNS myelin ligands. A) Representative Western blot/Rho immunoprecipitation in cell lysates and media of p75NTR, p75CTF, p75ICD, p75ECD, Rho-GTP, total Rho, phosphorylated EGFR (pEGFR), total EGFR, and ß-actin in mitotically arrested (with FDU) mixed adult rat DRG cultures after FGF2, TACE, PMA, and TIMP3 treatments, singly and in combination. B) Mean (±SD) integrated optical density of the protein bands from lysates and media seen in panel A and % reduction in Rho-GTP and pEGFR compared to control for treatments of DRG cultures with TACE, TACE + FGF2, PMA + FGF2, and TACE + FGF2 + TIMP3. ß-actin was used as a loading control. ***P < 0.0001.
