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Figure 2. Effects of MC1R isoforms on MITF and tyrosinase expressions. Levels of expression of MITF (A) and Tyr (B) transcripts in NHM that have been untransfected, mock transfected, transfected with pCMV-Script-MC1R317 or with pCMV-Script-MC1R350. Three independent measurements have been performed for each case. T tests were performed using GraphPad Prism software, and in each case P < 0.05. Bars represent SD. MC1R317 shows a significant positive action on MITF and Tyr expression whereas MC1R350 clearly hampers the expression of the 2 genes.
