Click on image to view larger version.
Figure 1. A) Immunodetection of MC1R350 by 
PEP20 in paraffin-embedded skin sections from Caucasian (C), Asian (A), and African American (AA) donors. Three different subjects were tested for each ethnic group. Representative fluorescence images of MC1R350 detected by FITC-coupled PEP20 (green, left column) and nuclei detected by PI (red, right column) in nonirradiated skin are shown for one subject from each ethnic group. B) The related fluorescence index values obtained with the Scion Image program are displayed on the graph in panel C. Typical images of immunodetection by N2–18 of total MC1R expression after irradiation with 1 MED UV dose. A positive signal was obtained for the three African American samples only. Melanocytes had a significant increase in expression 1 and 7 days after irradiation. D) Immunodetection of MC1R350 by PEP20 in paraffin-embedded skin sections of three Asian, Caucasian, and African American subjects. Typical fluorescence images of MC1R350 detected by Texas Red (red, left column) and nuclei detected by 4',6'-diam idino-2-phenylidole (blue, right column) in UV-irradiated skin. One representative Asian specimen is displayed. E) Graph of fluorescence index measurements in 3 Asian, Caucasian, and African American subjects after immunodetection of MC1R350 by PEP20. Data are shown for unirradiated (UI) skin as well as 7 min, 1 day, and 7 days after 1 MED UV exposure.
