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Figure 1. Representative experiment. a) PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of CXCL12 G801A polymorphism in 3 different patients. PCR product was digested with the restriction endonuclease MspI. 801G allele resulted in 2 fragments of 202 and 100 pb and 801A variant in 1 fragment of 302bp. b) Flow cytometric analysis of CXCR4 expression on AML leucoblast cells. These results were obtained from a patient with newly diagnosed acute myelomonocytic leukemia (FAB-M4 AML). CXCR4 expression was measured on leucoblast populations after setting a gate on side scatter (SSC)/anti-CD45 APC scatter graph (gate R1), completed with CD34 expression to optimize leucoblast gating (gate R2). Intensity of CXCR4 expression is shown as signal/noise ratio defined as geometric mean fluorescence intensity (MFI) of CXCR4-expressing cells (signal) divided by MFI of cells stained with IgG1 isotypic control antibody (Ab) (noise).
