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Figure 3. Physical analysis of meiotic DNA from yeast strains carrying various DMD inserts. DNA was extracted at 0, 8, and 24 h (indicated above each lane) after induction of meiosis and digested with either PvuII (A, B) or NsiI (C). An XhoI-BglII fragment from HIS4 (probe right in Fig. 2
) was used as a probe. Site I represents the endogenous DSB site at the HIS4 promoter. The additional DSB band in MSY14 is indicated.
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